Human IgG4 Monoklonaler Antikörper

Human IgG4 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A2

Kat-Nr. : 66408-1-PBS

Synonyme

human IgG heavy chain, IgG Fc, IGHG, IGHG4, 1A1A2

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  WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

  human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 66408-1-Ig (same clone as 66408-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66408-1-Ig (same clone as 66408-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66408-1-PBS bindet in WB, IHC, IF-P, ELISA Human IgG4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: immunoglobulin heavy constant gamma 4 (G4m marker)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: AL928742
• Gene symbol: IGHG4
• Gene ID (NCBI): 3503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.