Huntingtin Polyklonaler Antikörper

Huntingtin Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 27206-1-AP

Synonyme

HTT, HD, HD protein, Huntingtin, myristoylated N-terminal fragment, Huntington disease protein

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  IHC staining of human cerebellum using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cerebellum using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of rat cerebellum using 27206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 27206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Ratten-Cerebellum-Gewebe, Maushirngewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27206-1-AP bindet in IHC, IF-P, ELISA Huntingtin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Huntingtin fusion protein Ag25922
• Vollständiger Name: huntingtin
• Berechnetes Molekulargewicht: 348 kDa
• GenBank-Zugangsnummer: NM_002111
• Gene symbol: Huntingtin
• Gene ID (NCBI): 3064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HTT(huntingtin), also named HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington's disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders, and dementia.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Florian (Verified Customer) (03-22-2024)

Primary antibody was tested for ICC, where no specific signal was observed. The antibody therefore was not able to specifically stain Huntingtin aggregates. We then also proceeded to detection of a WB, which resulted in a strong non-specific band. As the theoretical epitope of this antibody is of great interest for us, we would be very looking forward for improvements in this regard.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: HEK293T