IA-2/PTPRN Monoklonaler Antikörper

IA-2/PTPRN Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human, Kaninchen

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D3F5

Kat-Nr. : 66045-1-PBS

Synonyme

IA-2, PTPRN, 1D3F5, ICA 512, ICA512-cleaved cytosolic fragment

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  WB analysis using 66045-1-Ig (same clone as 66045-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66045-1-Ig (same clone as 66045-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66045-1-PBS bindet in WB, IHC, IF/ICC, ELISA IA-2/PTPRN und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IA-2/PTPRN fusion protein Ag17909
• Vollständiger Name: protein tyrosine phosphatase, receptor type, N
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa, 106-115 kDa
• GenBank-Zugangsnummer: BC007713
• Gene symbol: IA-2/PTPRN
• Gene ID (NCBI): 5798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.