IARS2 Rekombinanter Antikörper

IARS2 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242219G4

Kat-Nr. : 84764-5-RR

Synonyme

242219G4, EC:6.1.1.5, IleRS, Isoleucine tRNA ligase, Isoleucine--tRNA ligase, mitochondrial

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  WB analysis using 84764-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84764-5-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocellular cancer using 84764-5-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocellular cancer using 84764-5-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84764-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84764-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 84764-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 84764-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84764-5-RR against Human IARS2 were performed. The affinity constant is 0.154 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: human hepatocellular cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84764-5-RR bindet in WB, IHC, IF/ICC, ELISA IARS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IARS2 fusion protein Ag10696
• Vollständiger Name: isoleucyl-tRNA synthetase 2, mitochondrial
• Berechnetes Molekulargewicht: 1012 aa, 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: BC010218
• Gene symbol: IARS2
• Gene ID (NCBI): 55699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.