IBA1 Monoklonaler Antikörper

IBA1 Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

25

CloneNo.

1C6A10

Rezensionen (1)

Kat-Nr. : 66827-1-Ig

Synonyme

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

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Galerie der Validierungsdaten

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  IHC staining of human brain using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of THP-1 using 66827-1-Ig

  1X10^6 THP-1 cells were intracellularly stained with 0.2 ug Anti-Human IBA1 (66827-1-Ig, Clone:1C6A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Gliomgewebe, humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 20 publications below

Produktinformation

66827-1-Ig bindet in WB, IHC, IF, ELISA IBA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IBA1 fusion protein Ag28236
• Vollständiger Name: allograft inflammatory factor 1
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC009474
• Gene symbol: AIF1
• Gene ID (NCBI): 199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Publikationen

Species	Application	Title
human	IHC	Nat Commun; Fatal iatrogenic cerebral β-amyloid-related arteritis in a woman treated with lecanemab for Alzheimer's disease; Authors - Elena Solopova
	IF	Cell Death Dis; ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis; Authors - Yaxuan Zhang
	IF	Oxid Med Cell Longev; Senkyunolide I Protects against Sepsis-Associated Encephalopathy by Attenuating Sleep Deprivation in a Murine Model of Cecal Ligation and Puncture.; Authors - Jian Xie
	IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	IHC,IF	Brain Pathol; Differential perivascular microglial activation in the deep white matter in vascular dementia developed post-stroke.; Authors - Yoshiki Hase
	IF	Mol Neurobiol; Hmox1 is Identified as a Ferroptosis Hub Gene and Associated with the M1 Type Microglia/Macrophage Polarization in Spinal Cord Injury: Bioinformatics and Experimental Validation; Authors - Yong Xuan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nuc (Verified Customer) (10-24-2023)

There is a clear band at 25 kD which is not the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: postmortem human frontal cortex

IBA1 Antibody Western Blot validation (1:1000 dilution) in postmortem human frontal cortex (Cat no:66827-1-Ig)