ICAM-1 Monoklonaler Antikörper

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• KD/KO Validated

ICAM-1 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

46

CloneNo.

2F9A8

Kat-Nr. : 60299-1-Ig

Synonyme

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

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Galerie der Validierungsdaten

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  WB analysis using 60299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60299-1-Ig

  WB result of ICAM1 antibody (60299-1-Ig, 1:50,000) with si-Control and si-ICAM1 transfected Raji cells. .

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  WB analysis of HeLa using 60299-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60299-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 60299-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 60299-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human lung cancer using 60299-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 60299-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Daudi using 60299-1-Ig

  1X10^6 Daudi cells were surface stained with 0.4 ug Anti-Human ICAM-1 (60299-1-Ig, Clone:2F9A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Daudi-Zellen, HeLa-Zellen, HepG2-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lungenkarzinomgewebe
• Erfolgreiche Detektion in FC: Daudi-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 32 publications below
• IHC: See 12 publications below
• IF: See 10 publications below

Produktinformation

60299-1-Ig bindet in WB, IHC, IF, FC, ELISA ICAM-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ICAM-1 fusion protein Ag8309
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC015969
• Gene symbol: ICAM1
• Gene ID (NCBI): 3383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

Publikationen

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB,IHC	Nat Commun; CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy; Authors - Xue Bai
human	WB, IF	EBioMedicine; Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.; Authors - Peng Zhou
	IF	Redox Biol; Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.; Authors - Zhuqing Li
	WB,IHC	Int J Biol Sci; Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury; Authors - Juan Zhang
human	IF	J Cell Biol; Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1.; Authors - Thomas Bessy

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