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ICAM-1 Monoklonaler Antikörper

ICAM-1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF

Konjugation

FITC Fluorescent Dye

CloneNo.

2F9A8

Kat-Nr. : FITC-60299

Synonyme

CD54, ICAM1, ICAM-1

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using FITC-conjugated ICAM-1 Monoclonal antibody (FITC-60299)

    IF Staining of human lung cancer using FITC-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using FITC-60299 (ICAM-1 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using FITC-conjugated ICAM-1 Monoclonal antibody (FITC-60299)

    IF Staining of human lung cancer using FITC-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using FITC-60299 (ICAM-1 antibody) at dilution of 1:50.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumanes Lungenkarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

FITC-60299 bindet in IF ICAM-1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ICAM-1 fusion protein Ag8309
Vollständiger Name intercellular adhesion molecule 1
Berechnetes Molekulargewicht 90 kDa
Beobachtetes Molekulargewicht 90 kDa
GenBank-ZugangsnummerBC015969
Gene symbol ICAM1
Gene ID (NCBI) 3383
Konjugation FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784). This antibody is FITC conjugated ICAM1 mouse monoclonal antibody (600299-1-Ig) which has passed in WB, IHC and FC detection.

Protokolle

Produktspezifische Protokolle
IF protocol for FITC ICAM-1 antibody FITC-60299Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Appl Bionics Biomech

Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway.

Authors - Song Yang
View Article