ICAM-1/CD54 Rekombinanter Antikörper

ICAM-1/CD54 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240725D10

Kat-Nr. : 83696-2-PBS

Synonyme

CD54, ICAM-1, Icam1

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  Sandwich ELISA standard curve of MP00678-2

  Sandwich ELISA standard curve of MP00678-2, Rat ICAM-1/CD54 Recombinant Matched Antibody Pair - PBS only. 83696-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1025. 83696-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00678-2

  Serum of sixteen rats was measured. The ICAM-1/CD54 concentration of detected samples was determined to be 15.3 ng/mL with a range of 12.9 - 20.5 ng/mL.

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  Sample test of MP00678-2

  Rat heart conditioned media (1 heart, 1-2 mm pieces) was cultured for 4 days in 30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The mean ICAM-1/CD54 concentration was determined to be 2,606.5 pg/mL in stimulated heart supernatant.
  Spleens from rats were removed, rinsed in PBS, and kept on ice. The tissue was homogenized using a tissue homogenizer and seeded into media containing DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL of streptomycin sulfate, and 50 ng/mL gentamicin solution. Cells were culture stimulated with 50 ng/mL of recombinant rat IL-2 and 50 ng/ml PHA for 3 days. The mean ICAM-1/CD54 concentration was determined to be 423.9 pg/mL in stimulated spleen supernatant.

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  Cytometric bead array standard curve of MP00678-2

  Cytometric bead array standard curve of MP00678-2, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-4-PBS. Detection antibody: 83696-2-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83696-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ICAM-1/CD54 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICAM-1/CD54 fusion protein Eg1025
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_012967.1
• Gene symbol: Icam1
• Gene ID (NCBI): 25464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.