ICAM-1/CD54 Rekombinanter Antikörper

ICAM-1/CD54 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240725C2

Kat-Nr. : 83696-3-PBS

Synonyme

CD54, ICAM-1, 240725C2, Icam1, ICAM-12

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  WB analysis using 83696-3-RR (same clone as 83696-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 83696-3-RR (same clone as 83696-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 83696-3-RR (same clone as 83696-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 83696-3-RR (same clone as 83696-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

• 

  IF Staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ICAM-1/CD54 antibody (83696-3-RR, Clone: 240725C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83696-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83696-3-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.09 nM.

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Geprüfte Anwendungen

Produktinformation

83696-3-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA ICAM-1/CD54 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICAM-1/CD54 fusion protein Eg1025
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: NM_012967.1
• Gene symbol: Icam1
• Gene ID (NCBI): 25464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).