ICAM-1/CD54 Rekombinanter Antikörper

ICAM-1/CD54 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240249D10

Kat-Nr. : 98049-1-RR

Synonyme

CD54, Icam-1, MALA-2, Icam1, ICAM 1

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  FC experiment of mouse splenocytes using 98049-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR, Clone:240249D10) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98049-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR, Clone:240249D10)(red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98049-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98049-1-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.49 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98049-1-RR bindet in FC ICAM-1/CD54 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: NM_010493
• Gene symbol: ICAM-1
• Gene ID (NCBI): 15894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).