ICAM-1/CD54 Rekombinanter Antikörper

ICAM-1/CD54 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240725C2

Kat-Nr. : 98060-1-RR

Synonyme

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

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• 

  FC experiment of rat splenocytes using 98060-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) (red) or isotype control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98060-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98060-1-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 0.347 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Ratten-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98060-1-RR bindet in FC ICAM-1/CD54 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_012967.1
• Gene symbol: Icam1
• Gene ID (NCBI): 25464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).