ICAM-1/CD54 Rekombinanter Antikörper

ICAM-1/CD54 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

240249D10

Kat-Nr. : PE-98049

Synonyme

CD54, Icam-1, 240249D10, ICAM 1, Icam1

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  FC experiment of mouse splenocytes using PE-98049

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3, and 0.1 ug PE Anti-Mouse ICAM-1/CD54 Rabbit RecAb (PE-98049, Clone: 240249D10) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-98049

  1x10^6 mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse ICAM-1/CD54 Rabbit RecAb (PE-98049, Clone: 240249D10) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-98049 bindet in FC ICAM-1/CD54 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICAM-1/CD54 fusion protein Eg0482
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: NM_010493
• Gene symbol: ICAM-1
• Gene ID (NCBI): 15894
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).