ICAM-2/CD102 Rekombinanter Antikörper

ICAM-2/CD102 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241387F11

Kat-Nr. : 84442-1-RR

Synonyme

ICAM-2, ICAM2, ICAM 2, CD102, 241387F11

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  WB analysis using 84442-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 84442-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 84442-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84442-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84442-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84442-1-RR against Mouse ICAM-2/CD102 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, bEnd.3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Mauslungengewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84442-1-RR bindet in WB, IHC, ELISA ICAM-2/CD102 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: intercellular adhesion molecule 2
• Berechnetes Molekulargewicht: 31kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: NM_010494.2
• Gene symbol: Icam2
• Gene ID (NCBI): 15896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.