ICAM-2/CD102 Rekombinanter Antikörper

ICAM-2/CD102 Rekombinant Antikörper für IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242237F8

Kat-Nr. : 84857-2-PBS

Synonyme

CD102, ICAM2, 242237F8, ICAM 2, ICAM-2

---

• 

  Sandwich ELISA standard curve of MP01646-1

  Sandwich ELISA standard curve of MP01646-1, Human ICAM2/CD102 Recombinant Matched Antibody Pair - PBS only. 84857-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2002. 84857-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01646-1

  Urine of four individual healthy human donors was measured. The ICAM2/CD102 concentration of detected samples was determined to be 14.2 ng/mL with a range of 6.9-18.8 ng/mL.

• 

  Sample test of MP01646-1

  Serum of four individual healthy human donors was measured. The ICAM2/CD102 concentration of samples was determined to be 425.2 ng/mL with a range of 346.3-500.1 ng/mL.

• 

  IF Staining of HUVEC using 84857-2-RR (same clone as 84857-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ICAM-2/CD102 antibody (84857-2-RR, Clone: 242237F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84857-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 84857-2-RR (same clone as 84857-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ICAM-2/CD102 antibody (84857-2-RR, Clone: 242237F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84857-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01646-1

  Cytometric bead array standard curve of MP01646-1, ICAM2/CD102 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84857-2-PBS. Detection antibody: 84857-1-PBS. Standard: Eg2002. Range: 0.625-80 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

84857-2-PBS bindet in IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ICAM-2/CD102 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICAM-2/CD102 fusion protein Eg2002
• Vollständiger Name: intercellular adhesion molecule 2
• Berechnetes Molekulargewicht: 31kDa
• GenBank-Zugangsnummer: NM_001099789.2
• Gene symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.