ICAM2/CD102 Polyklonaler Antikörper

ICAM2/CD102 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31950-1-AP

Synonyme

CD102, ICAM-2, ICAM 2, ICAM2, ICAM-2/CD102

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  WB analysis using 31950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31950-1-AP (ICAM2/CD102 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of bEnd.3 using 31950-1-AP

  IP result of anti-ICAM2/CD102 (IP:31950-1-AP, 4ug; Detection:31950-1-AP 1:4000) with bEnd.3 cells lysate 720 ug.

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  IHC staining of mouse spleen using 31950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31950-1-AP (ICAM2/CD102 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 31950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31950-1-AP (ICAM2/CD102 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: bEnd.3-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31950-1-AP bindet in WB, IHC, IP, ELISA ICAM2/CD102 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICAM2/CD102 fusion protein Eg1540
• Vollständiger Name: intercellular adhesion molecule 2
• Berechnetes Molekulargewicht: 31kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: NM_010494.2
• Gene symbol: Icam2
• Gene ID (NCBI): 15896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intercellular adhesion molecule 2 (ICAM2) is also named CD102. ICAM2 is a type I transmembrane glycoprotein containing two immunoglobulin-like domains, which are crucial to lymphocyte recirculation and mediate cell survival signals via pathways associated with LFA-1 (PMID: 2497351, PMID: 11825565). ICAM2 has been reported to be a mediator for a survival signal sufficient to block apoptosis by activation of the PI3K/AKT pathway (PMID: 11825565). ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.