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ICAM3/CD50 Rekombinanter Antikörper

ICAM3/CD50 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241417F3

Kat-Nr. : 84335-5-PBS

Synonyme

CDW50, ICAM3, 241417F3, CD50, ICAM 3

Filter:
  • Western Blot (WB) analysis of various lysates using ICAM3/CD50 Recombinant antibody (84335-5-RR)

    WB analysis using 84335-5-RR (same clone as 84335-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84335-5-RR (ICAM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84335-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ICAM3/CD50 Recombinant antibody (84335-5-RR)

    WB analysis of Jurkat using 84335-5-RR (same clone as 84335-5-PBS)

    WB result of ICAM3/CD50 antibody (84335-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells. This data was developed using the same antibody clone with 84335-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84335-5-RR

    Affinity and Kinetic Characterization of 84335-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84335-5-RR against Human ICAM3/CD50 were performed. The affinity constant is 24.7 nM.

Geprüfte Anwendungen

Produktinformation

84335-5-PBS bindet in WB, Indirect ELISA ICAM3/CD50 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name intercellular adhesion molecule 3
Berechnetes Molekulargewicht 547 aa, 60 kDa
Beobachtetes Molekulargewicht 110-130 kDa
GenBank-ZugangsnummerBC058903
Gene symbol ICAM3/CD50
Gene ID (NCBI) 3385
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.