ICAM3/CD50 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

ICAM3/CD50 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241417F3

Kat-Nr. : 84335-5-RR

Synonyme

CDW50, ICAM3, 241417F3, CD50, ICAM 3

---

• 

  WB analysis using 84335-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84335-5-RR (ICAM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 84335-5-RR

  WB result of ICAM3/CD50 antibody (84335-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells.

• 

  Affinity and Kinetic Characterization of 84335-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84335-5-RR against Human ICAM3/CD50 were performed. The affinity constant is 24.7 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Ramos-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84335-5-RR bindet in WB, ELISA ICAM3/CD50 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: intercellular adhesion molecule 3
• Berechnetes Molekulargewicht: 547 aa, 60 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC058903
• Gene symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.