ICAM3/CD50 Polyklonaler Antikörper

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ICAM3/CD50 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17699-1-PBS

Synonyme

CDW50, ICAM3, CD50, ICAM 3, ICAM R

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• 

  WB analysis of Jurkat using 17699-1-AP (same clone as 17699-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 17699-1-AP (same clone as 17699-1-PBS)

  WB result of ICAM3/CD50 antibody (17699-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 17699-1-AP (same clone as 17699-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 17699-1-AP (same clone as 17699-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 17699-1-AP (same clone as 17699-1-PBS)

  IP result of anti-ICAM3/CD50 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 17699-1-AP (same clone as 17699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 17699-1-AP (same clone as 17699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17699-1-PBS bindet in WB, IHC, IP, Indirect ELISA ICAM3/CD50 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICAM3/CD50 fusion protein Ag11955
• Vollständiger Name: intercellular adhesion molecule 3
• Berechnetes Molekulargewicht: 547 aa, 60 kDa
• Beobachtetes Molekulargewicht: 110-160 kDa, 60 kDa
• GenBank-Zugangsnummer: BC058903
• Gene symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572).