ICAM3/CD50 Rekombinanter Antikörper

ICAM3/CD50 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241418C10

Kat-Nr. : 84335-3-RR

Synonyme

CDW50, ICAM3, ICAM R, ICAM 3, CD50

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  IF Staining of Ramos using 84335-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using ICAM3 antibody (84335-3-RR, Clone: 241418C10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84335-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84335-3-RR against Human ICAM3/CD50 were performed. The affinity constant is 1.45 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Ramos-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84335-3-RR bindet in IF/ICC, ELISA ICAM3/CD50 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: intercellular adhesion molecule 3
• Berechnetes Molekulargewicht: 547 aa, 60 kDa
• GenBank-Zugangsnummer: BC058903
• Gene symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.