ICAM4 Monoklonaler Antikörper

ICAM4 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A9B9

Kat-Nr. : 67014-1-PBS

Synonyme

CD242, ICAM 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW

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  WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

  Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  WB analysis of human red blood cells using 67014-1-Ig (same clone as 67014-1-PBS)

  human red blood cells were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67014-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ICAM4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ICAM4 fusion protein Ag27628
• Vollständiger Name: intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
• Berechnetes Molekulargewicht: 271 aa, 29 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC029364
• Gene symbol: ICAM4
• Gene ID (NCBI): 3386
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.