ICOS/CD278 Polyklonaler Antikörper

ICOS/CD278 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13338-1-AP

Synonyme

ICOS, inducible T cell co stimulator, CVID1, CD278, AILIM

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  WB analysis of Jurkat using 13338-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 13338-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13338-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13338-1-AP (ICOS antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 13338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using ICOS antibody (13338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13338-1-AP bindet in WB, IHC, IF-P, ELISA ICOS/CD278 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICOS/CD278 fusion protein Ag4155
• Vollständiger Name: inducible T-cell co-stimulator
• Berechnetes Molekulargewicht: 199 aa, 23 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 45-50 kDa
• GenBank-Zugangsnummer: BC028210
• Gene symbol: ICOS
• Gene ID (NCBI): 29851
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13. Catalog#13338-1-AP recognizes two bands of 25-30 kDa and 45-50 kDa, and the additional 45-50 kDa band may be due to  surface disulfide-linked homodimeric glycoprotein.