ICOS/CD278 Rekombinanter Antikörper

ICOS/CD278 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242639D5

Kat-Nr. : 85035-2-PBS

Synonyme

242639D5, Activation-inducible lymphocyte immunomediatory molecule, Ailim, CCLP, CD278

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  WB analysis using 85035-2-RR (same clone as 85035-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85035-2-RR (ICOS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85035-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85035-2-RR (same clone as 85035-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85035-2-RR (ICOS/CD278 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85035-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85035-2-RR (same clone as 85035-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85035-2-RR (ICOS/CD278 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85035-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01745-2

  Cytometric bead array standard curve of MP01745-2, MOUSE ICOS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85035-2-PBS. Detection antibody: 85035-3-PBS. Standard: Eg3198. Range: 0.625-80 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85035-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA ICOS/CD278 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICOS/CD278 fusion protein Eg3198
• Vollständiger Name: inducible T-cell co-stimulator
• Berechnetes Molekulargewicht: 23kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_017480.2
• Gene symbol: Icos
• Gene ID (NCBI): 54167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13.