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ICOSLG/B7-H2/CD275 Rekombinanter Antikörper

ICOSLG/B7-H2/CD275 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240243G5

Kat-Nr. : 98058-1-PBS

Synonyme

CD275, B7-H2, Icosl, ICOS L, GL50

Filter:
  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant A (98058-1-RR)

    FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed. This data was developed using the same antibody clone with 98058-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant A (98058-1-RR)

    FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98058-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98058-1-RR

    Affinity and Kinetic Characterization of 98058-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98058-1-RR against Mouse ICOSLG/B7-H2/CD275 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

98058-1-PBS bindet in FC ICOSLG/B7-H2/CD275 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name icos ligand
Berechnetes Molekulargewicht36 kDa
GenBank-ZugangsnummerNM_015790.3
Gene symbol CD275
Gene ID (NCBI) 50723
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C.

Hintergrundinformationen

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).