ICOSLG/B7-H2/CD275 Rekombinanter Antikörper

ICOSLG/B7-H2/CD275 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240243G5

Kat-Nr. : 98058-1-RR

Synonyme

CD275, B7-H2, Icosl, ICOS L, GL50

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  FC experiment of mouse splenocytes using 98058-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98058-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98058-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98058-1-RR against Mouse ICOSLG/B7-H2/CD275 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98058-1-RR bindet in FC ICOSLG/B7-H2/CD275 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: icos ligand
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_015790.3
• Gene symbol: CD275
• Gene ID (NCBI): 50723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).