ICOSLG/B7-H2/CD275 Rekombinanter Antikörper

ICOSLG/B7-H2/CD275 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242241C8

Kat-Nr. : 98358-1-RR

Synonyme

B7H2, ICOSLG, B7 H2, B7-like protein Gl50, B7-related protein 1

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  FC experiment of human monocyte-derived mature dendritic cells using 98358-1-RR

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human ICOSLG/B7-H2/CD275 Rabbit RecAb (98358-1-RR, Clone: 242241C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98358-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98358-1-RR against Human ICOSLG/B7-H2/CD275 were performed. The affinity constant is 10.4 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human monocyte-derived mature dendritic cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98358-1-RR bindet in FC ICOSLG/B7-H2/CD275 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICOSLG/B7-H2/CD275 fusion protein Eg0008
• Vollständiger Name: inducible T-cell co-stimulator ligand
• Berechnetes Molekulargewicht: 33 kDa, 34 kDa
• GenBank-Zugangsnummer: BC064637
• Gene symbol: ICOSLG
• Gene ID (NCBI): 23308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).