ICOSLG/B7-H2/CD275 Rekombinanter Antikörper

ICOSLG/B7-H2/CD275 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

240243G5

Kat-Nr. : PE-98058

Synonyme

B7-H2, CD275, Icosl, 240243G5, B7 H2

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  FC experiment of mouse splenocytes using PE-98058

  1x10^6 mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2), and 0.1 ug PE Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit RecAb (PE-98058, Clone: 240243G5) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were not fixed.

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  FC experiment of mouse splenocytes using PE-98058

  1x10^6 mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit RecAb (PE-98058, Clone: 240243G5) (red) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-98058 bindet in FC ICOSLG/B7-H2/CD275 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICOSLG/B7-H2/CD275 fusion protein Eg0923
• Vollständiger Name: icos ligand
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_015790.3
• Gene symbol: CD275
• Gene ID (NCBI): 50723
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).