ICT1 Monoklonaler Antikörper

ICT1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F11E6

Kat-Nr. : 68898-3-Ig

Synonyme

1F11E6, 39S ribosomal protein L58, mitochondrial, DS1, DS-1, EC:3.1.1.29

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  WB analysis using 68898-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68898-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 68898-3-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 68898-3-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ICT1 antibody (68898-3-Ig, Clone: 1F11E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68898-3-Ig bindet in WB, IF/ICC, ELISA ICT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ICT1 fusion protein Ag33109
• Vollständiger Name: immature colon carcinoma transcript 1
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 21-24 kDa
• GenBank-Zugangsnummer: BC015335
• Gene symbol: ICT1
• Gene ID (NCBI): 3396
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.