ID3 Polyklonaler Antikörper

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• KD/KO Validated

ID3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10389-1-AP

Synonyme

1R21, bHLHb25, HEIR 1, HEIR1, ID3, Inhibitor of DNA binding 3

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Galerie der Validierungsdaten

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  WB analysis of Transfected HEK-293 using 10389-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10389-1-AP (ID3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10389-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10389-1-AP (ID3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

10389-1-AP bindet in WB, IHC, ELISA ID3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Nerz
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ID3 fusion protein Ag0588
• Vollständiger Name: inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
• Berechnetes Molekulargewicht: 13 aa, 5 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC003107
• Gene symbol: ID3
• Gene ID (NCBI): 3399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3, synonym: HEIR-1) is a member of the ID family of HLH proteins, which lacks a basic DNA-binding domain and inhibits transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Bcl-3 regulates TGFβ signaling by stabilizing Smad3 during breast cancer pulmonary metastasis.; Authors - Xi Chen
mouse	WB	Biomedicines; BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging.; Authors - Tingting Li
rat	IHC	Cancer Sci; Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.; Authors - Woo Gye-Hyeong GH
mink	WB	Life Sci; Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway.; Authors - Zhuo Wang ; KD Validated