ID4 Polyklonaler Antikörper

ID4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21803-1-PBS

Synonyme

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  WB analysis using 21803-1-AP (same clone as 21803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21803-1-AP (ID4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21803-1-PBS bindet in WB, IHC, Indirect ELISA ID4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ID4 fusion protein Ag16883
• Vollständiger Name: inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
• Berechnetes Molekulargewicht: 161 aa, 17 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC014941
• Gene symbol: ID4
• Gene ID (NCBI): 3400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ID4 (inhibitor of DNA binding 4) negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. (PMID: 19783986). ID4 levels dictate the stem cell state in mouse spermatogonia (PMID: 28087628). It is involved in the regulation of many cellular processes during both prenatal development and tumorigenesis. ID4 can be detected 25-30 kDa (PMID: 34108007, PMID: 31847356).