IDE Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IDE Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21728-1-PBS

Synonyme

---

• 

  WB analysis using 21728-1-AP (same clone as 21728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21728-1-AP (same clone as 21728-1-PBS)

  WB result of IDE antibody (21728-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A2780 using 21728-1-AP (same clone as 21728-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

  IP result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

21728-1-PBS bindet in WB, IHC, IP, Indirect ELISA IDE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDE fusion protein Ag16392
• Vollständiger Name: Abeta degrading protease
• Berechnetes Molekulargewicht: 1019 aa, 118 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC096336
• Gene symbol: IDE
• Gene ID (NCBI): 3416
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDE (INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.