IDE Monoklonaler Antikörper

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IDE Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E10G1

Kat-Nr. : 67106-1-PBS

Synonyme

Insulin-degrading enzyme, Insulinase, Insulin protease, EC:3.4.24.56, Abeta-degrading protease

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  WB analysis using 67106-1-Ig (same clone as 67106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67106-1-Ig (same clone as 67106-1-PBS)

  WB result of IDE antibody (67106-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67106-1-Ig (same clone as 67106-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 67106-1-Ig (same clone as 67106-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67106-1-Ig (same clone as 67106-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67106-1-Ig (same clone as 67106-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67106-1-Ig (same clone as 67106-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67106-1-Ig (same clone as 67106-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67106-1-Ig (same clone as 67106-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67106-1-Ig (same clone as 67106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67106-1-Ig (same clone as 67106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67106-1-Ig (IDE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67106-1-Ig (same clone as 67106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67106-1-Ig (IDE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67106-1-PBS bindet in WB, IHC, Indirect ELISA IDE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IDE fusion protein Ag17161
• Vollständiger Name: Abeta degrading protease
• Berechnetes Molekulargewicht: 1019 aa, 118 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC096336
• Gene symbol: IDE
• Gene ID (NCBI): 3416
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).