IDH1 Polyklonaler Antikörper

IDH1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23309-1-AP

Synonyme

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD

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  WB analysis using 23309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23309-1-AP (IDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 23309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23309-1-AP (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 23309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23309-1-AP (IDH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

23309-1-AP bindet in WB, IHC, ELISA IDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDH1 fusion protein Ag19749
• Vollständiger Name: isocitrate dehydrogenase 1 (NADP+), soluble
• Berechnetes Molekulargewicht: 414 aa, 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC012846
• Gene symbol: IDH1
• Gene ID (NCBI): 3417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. Its subunit structure is homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ). This antibody is specific to IDH1.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation.; Authors - Haiying Zhang
human	WB	Med Oncol; Isocitrate dehydrogenase gene mutations and 2-hydroxyglutarate accumulation in esophageal squamous cell carcinoma.; Authors - Keisuke Miyake
human	WB	Antioxidants (Basel); Progranulin Deficiency Induces Mitochondrial Dysfunction in Frontotemporal Lobar Degeneration with TDP-43 Inclusions; Authors - Guiomar Rodríguez-Periñán