IDH1 Rekombinanter Antikörper

IDH1 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

6L18

Kat-Nr. : 81176-1-RR

Synonyme

IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase

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  WB analysis using 81176-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81176-1-RR (IDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 81176-1-RR

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 81176-1-RR (IDH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 81176-1-RR

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 81176-1-RR (IDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 81176-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH1 Recombinant antibody (81176-1-RR, Clone:6L18) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81176-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81176-1-RR against Human IDH1 were performed. The affinity constant is 17.5 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81176-1-RR bindet in WB, IHC, FC (Intra), ELISA IDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IDH1 fusion protein Ag19749
• Vollständiger Name: isocitrate dehydrogenase 1 (NADP+), soluble
• Berechnetes Molekulargewicht: 414 aa, 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC012846
• Gene symbol: IDH1
• Gene ID (NCBI): 3417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).