IDH3B Monoklonaler Antikörper

IDH3B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11A9

Kat-Nr. : 68199-1-Ig

Synonyme

FLJ11043, H IDHB, IDH3B, RP46

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  WB analysis using 68199-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68199-1-Ig (IDH3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Jurkat-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Hausschwein-Herzgewebe, Kaninchenhirngewebe, Kaninchenherzgewebe, Rattenhirngewebe, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68199-1-Ig bindet in WB, ELISA IDH3B und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IDH3B fusion protein Ag8693
• Vollständiger Name: isocitrate dehydrogenase 3 (NAD+) beta
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC001960
• Gene symbol: IDH3B
• Gene ID (NCBI): 3420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).