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IDH3B Monoklonaler Antikörper

IDH3B Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11A9

Kat-Nr. : 68199-1-PBS

Synonyme

FLJ11043, H IDHB, IDH3B, RP46

Filter:
  • Western Blot (WB) analysis of various lysates using IDH3B Monoclonal antibody (68199-1-Ig)

    WB analysis using 68199-1-Ig (same clone as 68199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68199-1-Ig (IDH3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68199-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68199-1-PBS bindet in WB, Indirect ELISA IDH3B und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen IDH3B fusion protein Ag8693
Vollständiger Name isocitrate dehydrogenase 3 (NAD+) beta
Berechnetes Molekulargewicht 42 kDa
Beobachtetes Molekulargewicht39-42 kDa
GenBank-ZugangsnummerBC001960
Gene symbol IDH3B
Gene ID (NCBI) 3420
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).