IDI1 Polyklonaler Antikörper

IDI1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11166-2-PBS

Synonyme

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  WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11166-2-AP (same clone as 11166-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

  IP result of anti-IDI1 (IP:11166-2-AP, 4ug; Detection:11166-2-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11166-2-PBS bindet in WB, IHC, IP, Indirect ELISA IDI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDI1 fusion protein Ag1636
• Vollständiger Name: isopentenyl-diphosphate delta isomerase 1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC019227
• Gene symbol: IDI1
• Gene ID (NCBI): 3422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDI1(Isopentenyl-diphosphate Delta-isomerase 1) is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). It belongs to the IPP isomerase type 1 family. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 26 kDa and 32 kDa.