IDO1 Monoklonaler Antikörper

IDO1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

CloneNo.

3G2G11

Kat-Nr. : 66528-1-Ig

Synonyme

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

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  WB analysis using 66528-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66528-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66528-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66528-1-Ig (IDO1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 66528-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using IDO1 antibody (66528-1-Ig, Clone: 3G2G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, mit IFN-gamma behandelte HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 22 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Produktinformation

66528-1-Ig bindet in WB, IHC, IF/ICC, ELISA IDO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IDO1 fusion protein Ag3953
• Vollständiger Name: indoleamine 2,3-dioxygenase 1
• Berechnetes Molekulargewicht: 403 aa, 45 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC027882
• Gene symbol: IDO1
• Gene ID (NCBI): 3620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Publikationen

Species	Application	Title
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
mouse	WB,IF	J Gastroenterol; Indoleamine 2,3-dioxygenase 1-mediated iron metabolism in macrophages contributes to lipid deposition in nonalcoholic steatohepatitis; Authors - Chaofeng Wu
human,mouse	WB,IHC,IF	Pharmacol Res; IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.; Authors - Xin Fang
mouse	WB	ACS Chem Neurosci; 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Induced Parkinson's Disease in Mouse: Potential Association between Neurotransmitter Disturbance and Gut Microbiota Dysbiosis.; Authors - Yuanhui Zhu
human	WB	Chin J Nat Med; Panax notoginseng saponins prevent colitis-associated colorectal cancer via inhibition IDO1 mediated immune regulation.; Authors - Xue-Ming Li
mouse	WB	Chin J Integr Med; Combination of Geniposide and Eleutheroside B Exerts Antidepressant-like Effect on Lipopolysaccharide-Induced Depression Mice Model.; Authors - Bo Zhang