IDO1 Monoklonaler Antikörper

IDO1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G2G11

Kat-Nr. : 66528-1-PBS

Synonyme

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

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  WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66528-1-Ig (same clone as 66528-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66528-1-Ig (same clone as 66528-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66528-1-Ig (IDO1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using IDO1 antibody (66528-1-Ig, Clone: 3G2G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66528-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IDO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IDO1 fusion protein Ag3953
• Vollständiger Name: indoleamine 2,3-dioxygenase 1
• Berechnetes Molekulargewicht: 403 aa, 45 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC027882
• Gene symbol: IDO1
• Gene ID (NCBI): 3620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).