IDO1 Rekombinanter Antikörper

IDO1 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241110C8

Kat-Nr. : 84063-1-PBS

Synonyme

241110C8, EC:1.13.11.52, IDO, IDO 1, IDO-1

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  WB analysis of HeLa using 84063-1-RR (same clone as 84063-1-PBS)

  IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 84063-1-RR (IDO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84063-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP00982-3

  Serum of fifteen individual healthy human donors was measured. The human IDO1 concentration of detected samples was determined to be 8.21 ng/mL with a range of ND - 41.58 ng/mL. (ND = Non-detectable)

• 

  Sandwich ELISA sample test of MP00982-3

  A549 cells were cultured unstimulated or stimulated with 300 μg/mL IFN-gamma for 5 days. The mean IDO1 concentration was determined to be 0.30 ng/mL in unstimulated A549 supernatant, 1.19 ng/mL in IFN-gamma stimulated A549 supernatant.

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  Sandwich ELISA standard curve of MP00982-3

  Sandwich ELISA standard curve of MP00982-3, Human IDO1 Recombinant Matched Antibody Pair - PBS only. 84063-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3953. 84063-4-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

• 

  FC experiment of HeLa using 84063-1-RR (same clone as 84063-1-PBS)

  1x10^6 IFN-gamma treated HeLa cells were intracellularly stained with 0.25 ug Ido1 Recombinant Antibody (84063-1-RR, Clone:241110C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84063-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00982-3

  Cytometric bead array standard curve of MP00982-3, IDO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84063-1-PBS. Detection antibody: 84063-4-PBS. Standard: Ag3953. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 84063-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84063-1-PBS against Human IDO1 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 84063-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84063-1-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84063-1-PBS bindet in WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA IDO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IDO1 fusion protein Ag3953
• Vollständiger Name: indoleamine 2,3-dioxygenase 1
• Berechnetes Molekulargewicht: 403 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC027882
• Gene symbol: IDO1
• Gene ID (NCBI): 3620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).