IDUA Polyklonaler Antikörper

IDUA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 55158-1-AP

Synonyme

Alpha L iduronidase, IDA, IDUA, iduronidase, alpha L, MPS1

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  WB analysis using 55158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55158-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 55158-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55158-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

55158-1-AP bindet in WB, IP, IHC, ELISA IDUA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: iduronidase, alpha-L-
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: NM_000203
• Gene symbol: IDUA
• Gene ID (NCBI): 3425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDUA belongs to the glycosyl hydrolase 39 family. It hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H)( also known as Hurler syndrome), MPS1H/S(also known as Hurler-Scheie syndrome) and MPS1S(also known as Scheie syndrome), which are rare lysosomal storage diseases. This antibody is specific to IDUA.

Publikationen

Species	Application	Title
human	WB	Mol Ther Methods Clin Dev; Liver-directed gene therapy corrects neurologic disease in a murine model of mucopolysaccharidosis type I-Hurler.; Authors - Xiu Jin
	IP	Mol Cell Biochem; Why SNP rs3755955 is associated with human bone mineral density? A molecular and cellular study in bone cells.; Authors - Pei He
human	WB,IHC	Front Immunol; Leveraging diverse cell-death related signature predicts the prognosis and immunotherapy response in renal clear cell carcinoma; Authors - Zhengqi Wu