IDUA Rekombinanter Antikörper

IDUA Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251274F3

Kat-Nr. : 86499-1-PBS

Synonyme

Alpha-L-iduronidase, EC:3.2.1.76, MPS1

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  WB analysis using 86499-1-RR (same clone as 86499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86499-1-RR (IDUA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86499-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02673-1

  The mean IDUA concentration was determined to be 6.07 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02673-1

  Sandwich ELISA standard curve of MP02673-1, Human IDUA Recombinant Matched Antibody Pair - PBS only. 86499-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30658. 86499-2-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

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  Affinity and Kinetic Characterization of 86499-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86499-1-RR against Human IDUA were performed. The affinity constant is 2.20 nM.

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Geprüfte Anwendungen

Produktinformation

86499-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA IDUA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IDUA fusion protein Ag30658
• Vollständiger Name: iduronidase, alpha-L-
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73-75 kDa
• GenBank-Zugangsnummer: NM_000203
• Gene symbol: IDUA
• Gene ID (NCBI): 3425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Iduronidase (L-iduronidase, alpha-L-iduronidase, laronidase) is an enzyme with the systematic name glycosaminoglycan alpha-L-iduronohydrolase. This enzyme catalyzes the hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. It is a glycoprotein enzyme found in the lysosomes of cells. It is involved in the degeneration of glycosaminoglycans such as dermatan sulfate and heparan sulfate. The enzyme acts by hydrolyzing the terminal alpha-L-iduronic acid residues of these molecules, degrading them (PMID: 4993544,30407). A deficiency in the IDUA protein is associated with mucopolysaccharidoses (MPS). MPS, a type of lysosomal storage disease, is typed I through VII. In this syndrome, glycosaminoglycans accumulate in the lysosomes and cause substantial disease in many different tissues of the body. IDUA mutations result in the MPS 1 phenotype, which is inherited in an autosomal recessive fashion. The defective alpha-L-iduronidase results in an accumulation of heparan and dermatan sulfate within phagocytes, endothelium, smooth muscle cells, neurons, and fibroblasts. Prenatal diagnosis of this enzyme deficiency is possible (PMID:8242073).