IFFO1 Polyklonaler Antikörper

IFFO1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16041-1-PBS

Synonyme

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  WB analysis of mouse brain using 16041-1-AP (same clone as 16041-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 16041-1-AP (same clone as 16041-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16041-1-AP (same clone as 16041-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 16041-1-AP (same clone as 16041-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16041-1-AP (same clone as 16041-1-PBS)

  Immunofluorescent analysis of Hela cells, using IFFO1 antibody 16041-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16041-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IFFO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFFO1 fusion protein Ag8915
• Vollständiger Name: intermediate filament family orphan 1
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC004384
• Gene symbol: IFFO1
• Gene ID (NCBI): 25900
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFFO1, also named as IFFO and Tumor antigen HOM-TES-103, is a member of the intermediate filament family which consists of primordial components of the cytoskeleton and nuclear envelope that are mainly distributed in the nucleus, followed by the cytoskeleton and cytoplasm. IFFO1 in the nucleus was identified to combine with both XRCC4 and the nuclear lamina protein lamin A/C to be involved in nonhomologous DNA end joining (NHEJ). The expression of IFFO1 is associated with tumor progression. IFFO1 could be used as a potential marker to differentiate malignant ascites cell-free tumor DNA (cftDNA) of ovarian cancer from benign controls.