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IFI16 Polyklonaler Antikörper

IFI16 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29280-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using IFI16 Polyclonal antibody (29280-1-AP)

    WB analysis using 29280-1-AP (same clone as 29280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29280-1-AP (IFI16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using IFI16 Polyclonal antibody (29280-1-AP)

    IHC staining of human renal cell carcinoma using 29280-1-AP (same clone as 29280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using IFI16 Polyclonal antibody (29280-1-AP)

    IHC staining of human renal cell carcinoma using 29280-1-AP (same clone as 29280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IFI16 Polyclonal antibody (29280-1-AP)

    IF Staining of HeLa using 29280-1-AP (same clone as 29280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI16 antibody (29280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29280-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IFI16 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IFI16 fusion protein Ag30297
Vollständiger Name interferon, gamma-inducible protein 16
Berechnetes Molekulargewicht 729 aa, 82 kDa
Beobachtetes Molekulargewicht85-95 kDa
GenBank-ZugangsnummerBC017059
Gene symbol IFI16
Gene ID (NCBI) 3428
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)