IFI16 Monoklonaler Antikörper

IFI16 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C10D7

Kat-Nr. : 67790-1-PBS

Synonyme

Ifi 16, IFI16, IFNGIP1, PYHIN2

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  WB analysis using 67790-1-Ig (same clone as 67790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67790-1-Ig (IFI16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  WB analysis using 67790-1-Ig (same clone as 67790-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67790-1-Ig (IFI16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67790-1-Ig (same clone as 67790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67790-1-Ig (same clone as 67790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67790-1-Ig (same clone as 67790-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI16 antibody (67790-1-Ig, Clone: 1C10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67790-1-PBS bindet in WB, IHC, IF/ICC, ELISA IFI16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFI16 fusion protein Ag30314
• Vollständiger Name: interferon, gamma-inducible protein 16
• Berechnetes Molekulargewicht: 729 aa, 82 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC017059
• Gene symbol: IFI16
• Gene ID (NCBI): 3428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)