IFI35 Rekombinanter Antikörper

IFI35 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240063C6

Kat-Nr. : 83160-2-PBS

Synonyme

240063C6, Ifi 35, Ifi-35, IFP 35, IFP35

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  IF Staining of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI35 antibody (83160-2-RR, Clone: 240063C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83160-2-RR (same clone as 83160-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00191-2

  Cytometric bead array standard curve of MP00191-2, IFI35 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83160-2-PBS. Detection antibody: 83160-1-PBS. Standard: Ag34653. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83160-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83160-2-PBS against Human IFI35 was performed. The affinity constant is 1.93 nM.

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  Affinity and Kinetic Characterization of 83160-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83160-2-RR against Human IFI35 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83160-2-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA IFI35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFI35 fusion protein Ag34653
• Vollständiger Name: interferon-induced protein 35
• Berechnetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC001356
• Gene symbol: IFI35
• Gene ID (NCBI): 3430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.