IFIH1/MDA5 Monoklonaler Antikörper

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• KD/KO Validated

IFIH1/MDA5 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3D8B11

Kat-Nr. : 66770-1-PBS

Synonyme

IFIH1, MDA5, 3D8B11, CADM-140 autoantigen, Clinically amyopathic dermatomyositis autoantigen 140 kDa

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  WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

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  WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66770-1-Ig (same clone as 66770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66770-1-Ig (same clone as 66770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66770-1-Ig (same clone as 66770-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug IFIH1/MDA5 Monoclonal antibody (66770-1-Ig, Clone:3D8B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66770-1-PBS bindet in WB, IHC, FC (Intra), ELISA IFIH1/MDA5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFIH1/MDA5 fusion protein Ag17448
• Vollständiger Name: interferon induced with helicase C domain 1
• Berechnetes Molekulargewicht: 1025 aa, 117 kDa
• Beobachtetes Molekulargewicht: 117-125 kDa
• GenBank-Zugangsnummer: BC111750
• Gene symbol: IFIH1
• Gene ID (NCBI): 64135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).