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IFITM1-Specific Monoklonaler Antikörper
IFITM1-Specific Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
5B5E2
Kat-Nr. : 60074-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | TF-1-Zellen, HepG2-Zellen, HL-60-Zellen, humanes Hodengewebe, K-562-Zellen | 
| Erfolgreiche IP | HepG2-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Lymphomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | K-562-Zellen, Jurkat-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:200-1:1000 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 14 publications below | 
| WB | See 66 publications below | 
| IHC | See 3 publications below | 
| IF | See 12 publications below | 
| IP | See 1 publications below | 
| FC | See 1 publications below | 
| CoIP | See 1 publications below | 
Produktinformation
60074-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA IFITM1-Specific und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus | 
| Wirt / Isotyp | Maus / IgG2a | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | IFITM1-Specific fusion protein Ag2320 | 
| Vollständiger Name | interferon induced transmembrane protein 1 (9-27) | 
| Berechnetes Molekulargewicht | 14 kDa | 
| Beobachtetes Molekulargewicht | 14-17 kDa | 
| GenBank-Zugangsnummer | BC000897 | 
| Gene symbol | IFITM1 | 
| Gene ID (NCBI) | 8519 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen | 
| IHC protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladenl | 
| IF protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen | 
| IP protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Science IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise. | ||
| Cell The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. | ||
| Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells | ||
| Sci Transl Med Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition. | ||
| Cell Host Microbe Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
 | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Claudia (Verified Customer) (06-20-2022) | The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines. 
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