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  • KD/KO Validated

IFITM1-Specific Monoklonaler Antikörper

IFITM1-Specific Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(77)

CloneNo.

5B5E2

Kat-Nr. : 60074-1-Ig

Synonyme

IFITM1, 5B5E2, 9 27, 927, CD225



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTF-1-Zellen, HepG2-Zellen, HL-60-Zellen, humanes Hodengewebe, K-562-Zellen
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IHChumanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCK-562-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60074-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA IFITM1-Specific und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen IFITM1-Specific fusion protein Ag2320
Vollständiger Name interferon induced transmembrane protein 1 (9-27)
Berechnetes Molekulargewicht 14 kDa
Beobachtetes Molekulargewicht 14-17 kDa
GenBank-ZugangsnummerBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
IHC protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladenl
IF protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
IP protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Science

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Authors - Julian Buchrieser
humanWB

Cell

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Authors - Abraham L Brass
humanWB

Nat Microbiol

Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells

Authors - Yinggui Yang
humanWB

Cell Stem Cell

PMID: 30416070

Authors - Carolina Petrillo
humanWB

Sci Transl Med

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Authors - Mohammed Selman
humanWB

Cell Host Microbe

Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.

Authors - Toshana L Foster
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:400 (immunofluorescence), 1:20.000 (Western blot)
  • Cell Tissue Type: Permanent cell lines