IFITM2/3 Monoklonaler Antikörper

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• KD/KO Validated

IFITM2/3 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E8D12

Kat-Nr. : 66081-1-PBS

Synonyme

IFITM3, 1 8U, 2E8D12, DSPA2b, Interferon-induced transmembrane protein 3

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  WB analysis using 66081-1-Ig (same clone as 66081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66081-1-Ig (same clone as 66081-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IFITM2/3 antibody (66081-1-Ig, Clone: 2E8D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66081-1-PBS bindet in WB, IHC, IF/ICC, ELISA IFITM2/3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFITM2/3 fusion protein Ag17863
• Vollständiger Name: interferon induced transmembrane protein 3 (1-8U)
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC070243
• Gene symbol: IFITM3
• Gene ID (NCBI): 10410
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.