IFITM3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IFITM3 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (7)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

124

Rezensionen (2)

Kat-Nr. : 11714-1-AP

Synonyme

1 8U, Fragilis, IFITM3, IP15

---

Galerie der Validierungsdaten

• 

  WB analysis using 11714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11714-1-AP

  IP result of anti-IFITM3(IP:11714-1-AP, 4ug; Detection:11714-1-AP 1:4000) with HepG2 cells lysate 960 ug.

• 

  IF Staining of HeLa using 11714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFITM3 antibody (11714-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 11714-1-AP

  1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, THP-1-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 34 publications below
• WB: See 100 publications below
• IHC: See 7 publications below
• IF: See 27 publications below
• IP: See 5 publications below
• FC: See 7 publications below
• CoIP: See 2 publications below

Produktinformation

11714-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA IFITM3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Maus, Ratte, Ziege, Südliche Grünmeerkatze
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFITM3 fusion protein Ag2285
• Vollständiger Name: interferon induced transmembrane protein 3 (1-8U)
• Berechnetes Molekulargewicht: 133 aa, 15 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC006794
• Gene symbol: IFITM3
• Gene ID (NCBI): 10410
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Publikationen

Species	Application	Title
human	WB	Science; A human apolipoprotein L with detergent-like activity kills intracellular pathogens.; Authors - Ryan G Gaudet
human,mouse	WB	Cell; A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.; Authors - Noémie Alphonse
human	WB	Nature; IFITM3 restricts the morbidity and mortality associated with influenza.; Authors - Everitt AR
mouse	IHC	Science; T cell memory. Skin-resident memory CD8⁺ T cells trigger a state of tissue-wide pathogen alert.; Authors - Silvia Ariotti
human	IF	Cell Stem Cell; Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids.; Authors - Galina Popova
human	WB,IF	Cell Stem Cell; PMID: 30416070; Authors - Carolina Petrillo ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Thomas (Verified Customer) (06-29-2022)

Works fine in WB and IF

• Applications: Western Blot, Immunofluorescence

FH

Xiuqing (Verified Customer) (03-21-2022)

worked very well for western blot

• Applications: Western Blot
• Cell Tissue Type: MARC-145 cells, H1299 cells

Verwandte Produkte

IFITM2 Polyclonal antibody

12769-1-AP

IFITM1-Specific Monoclonal antibody

60074-1-Ig

IFITM2-specific Monoclonal antibody

66137-1-Ig

Osteopontin Polyclonal antibody

22952-1-AP

NAMPT/PBEF Polyclonal antibody

11776-1-AP

IFITM1 Polyclonal antibody

11727-3-AP

IFN Gamma Polyclonal antibody

15365-1-AP