IFN alpha 2A Monoklonaler Antikörper

IFN alpha 2A Monoklonal Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

3C7G3

Kat-Nr. : 68665-1-PBS

Synonyme

IFN alpha 2, IFNA2, IFN alpha2, IFN-alpha 2, IFN-alpha-2

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  Sandwich ELISA standard curve of MP50005-1

  Sandwich ELISA standard curve of MP50005-1, IFNA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69008-1-PBS. Detection antibody: 68665-1-PBS. Standard: HZ-1066. Range: 7.8-500 pg/mL.

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  Sample test of MP50005-1

  Serum of six individual healthy human donors was measured. The IFN alpha 2 concentration of detected samples was determined to be 66.5 pg/mL with a range of 29.0-185.5 pg/mL.

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  Sample test of MP50005-1

  Human peripheral blood mononuclear cells (PBMC) was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL poly I:C and Lipofectamine 2000 (LF2K) for 1 day. An aliquot of the cell culture supernatant was removed, the mean IFN alpha 2 concentration was undetectable in unstimulated PBMC supernatant, 159.7 pg/mL in poly I:C and LF2K stimulated PBMC supernatant.

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Geprüfte Anwendungen

Produktinformation

68665-1-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test IFN alpha 2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFN alpha 2A fusion protein HZ-1066
• Vollständiger Name: interferon, alpha 2
• Gene symbol: IFN alpha2
• Gene ID (NCBI): 3440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.